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. 2017 May 17;8:565. doi: 10.3389/fimmu.2017.00565

Figure 4.

Figure 4

Expansion and restimulation increases resolution in the phenotypical profile of commensal-specific T cells. Cytokine-producing cells of porcine PBMC were directly analyzed ex vivo after Candida albicans restimulation, gated as described in Figure 1A and plotted as panel (A) cytokine-producing cells of total CD4+ T cells or panel (B) cytokine/CD154+ coproducing cells of CD4+ T cells (n = 10–11). PBMCs were further expanded for C. albicans antigen and analyzed for cytokine/CD154+ expression following restimulation with Cand-primed monocyte-derived dendritic cell (MoDC) (40 µg/ml). Panel (C) illustrates representative cytokine/CD154 analysis of expanded C. albicans-specific T cells restimulated with either umprimed (w/o) or C. albicans-primed MoDC. Cytokine analysis of C. albicans-expanded T cells are summarized in panel (D) (TNF-α: n = 10, unpaired t-test, p = 0.0166, IFN-γ: w/o n = 6, Cand n = 8, Mann–Whitney test, p = 0.0047, interleukin-17 w/o n = 4, Cand n = 8, Mann–Whitney test, p = 0.073).