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. 2005 Jan;25(2):779–788. doi: 10.1128/MCB.25.2.779-788.2005

FIG. 8.

FIG. 8.

Growth and DNA repair characteristics of Brctx-null and wild-type cells. (A) Growth curve of P2 MEFs derived from Brctx-null and wild-type embryonic day 13.5 embryos. Symbols: ▪, wild-type (WT) cells; ▴, Brctx-null cells. Cell counts were performed in duplicate at intervals of 24 h. The data represent means ± the standard deviations of three independent experiments. (B) Role of Brctx in DNA repair. The data for MEF experiments represent the mean ± the standard errors of the mean for MTT assays in 96-well plate format. Treatments: I, UV irradiation; II, IR; III, MMS; IV, MMC. The data were normalized to untreated cells of the same genotype. (C) BRCTx-EGFP fusion proteins do not relocalize in response to DNA damage. Stable hamster V79 cells expressing BRCTx-EGFP fusion proteins were treated with DNA-damaging agents as indicated. At 24 h after exposure, cells were stained with DAPI and examined by immunofluorescence. The data are representative of two independent experiments.