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. 2017 May 17;11:273. doi: 10.3389/fnins.2017.00273

Figure 5.

Figure 5

Exosomes from NSC-34 motor neurons (MNs) mutated in G93A (mSOD1) lead to delayed upregulation of the receptors TREM2, RAGE and TLR4 in N9 microglia. N9 cells were incubated for 2, 4, and 24 h with exosomes (Exos) from wild-type (wt) NSC-34 MNs and mSOD1 NSC-34 MNs (N9+wt Exos and N9+mSOD1 Exos, respectively), as indicated in methods. Non-treated cells were considered as control. Gene expression of (A) triggering receptor expressed on myeloid cells 2 (TREM2), (B) Receptor for Advanced Glycation Endproducts (RAGE) and (C) Toll-like receptor-4 (TLR4) was determined by qRT-PCR in total RNA. Results are mean (± SEM) from at least five independent experiments and are expressed as fold change relatively to non-treated N9 microglia. Differences between the three different groups at each time point were obtained by one-way ANOVA followed by Bonferroni post-hoc correction. *p < 0.05 and **p < 0.01 vs. non-treated cells; #p < 0.05 and ##p < 0.01 vs. treatment with exosomes from wt NSC-34 MNs.