Figure 7.

Early decreased expression of calming microRNAs (miR-124 and miR-146a) is indicative of N9 microglia M1 phenotype, but their increase together with that of miR-155 suggests the coexistence of multiple activated phenotypes at 24 h. N9 microglial cells were incubated for 2, 4, and 24 h with exosomes (Exos) from wild-type (wt) NSC-34 MNs and mSOD1 NSC-34 MNs (N9+wt Exos and N9+mSOD1 Exos, respectively), as indicated in methods. Non-treated cells were considered as control. (A–C) Relative miR-124, miR-146a, and miR-155 levels were determined by qRT-PCR in total RNA. Results are mean (± SEM) from eight independent experiments and are expressed as fold changes relatively to non-treated microglia. Differences between the three different groups at each time point were obtained by one-way ANOVA followed by Bonferroni post-hoc correction. ^*p < 0.05 and ^**p < 0.01 vs. non-treated cells; ^##p < 0.01 vs. treatment with exosomes from wt NSC-34 MNs.