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. 2005 Jan;25(2):789–796. doi: 10.1128/MCB.25.2.789-796.2005

FIG. 2.

FIG. 2.

(A) Western blot analysis with anti-Ii antibody (In-1) reveals no difference in Ii isoforms p41 and p31 and the degradation product p10 in thymic stromal cells of Prss16−/− mice compared to those of wild-type mice. The genotype of each animal is indicated. (B) MHC class II surface expression on BP-1+CD4−CD11c− cells. Shown are means and standard errors of the means. Wild-type and heterozygous animals were grouped together (Prss16+/?), cTEC were identified by gating on low-density thymic cells that were BP-1+CD4−CD11c−, and surface expression of MHC class II was estimated by mean fluorescence intensity (MFI). No significant difference was detected between Prss16−/− mice and their littermate controls (n = 8 per group). (C) Representative results of flow cytometry of low-density thymic cells stained for BP-1, CD4, CD11c, and MHC class II.