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. 2005 Jan;25(2):789–796. doi: 10.1128/MCB.25.2.789-796.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — The percentages and total numbers of CD4⁺-, CD8⁺-, and CD4⁺CD8⁺-T-cell populations are similar for Prss16⁻^/⁻ and Prss16^+/+ mice. Thymocytes (A) and splenocytes (B) from Prss16⁻^/⁻ and Prss16^+/+ littermates were stained with anti-CD4 and anti-CD8 monoclonal antibodies (MAb). The percentage of the total number of T cells in each population is indicated. The total numbers of T cells in the thymus and spleen of Prss16⁻^/⁻ mice did not differ significantly. (C) The percentage of CD3⁻ thymocytes expressing CD44, CD25, or both did not differ significantly between Prss16⁻^/⁻ (solid bar) and Prss16^+/+ (hatched bar) littermates. Thymocytes were stained with anti-CD3, anti-CD44, and anti-CD25 MAb. The CD3⁻ population was gated for analysis of CD44 and CD25. The percentage of the total number of T cells in each population is indicated.