TABLE 2.

Primers

Primer name	Primer sequencea
C11sen1	5′-GCATCTCGAGATGCAGTTTTGTCCTACTTGTGG AA; XhoI
C11ant1	5′-CGAGGATCCCTAATTCTCACGCCATTGAAATTTGG; BamHI
C11ant2	5′-CGAGGATCCCTAATTCTCACGCCATTGAAATTTGC; BamHI
Seq1	5′-TCAATCTCATTCTCACTTTCTGAC
C11ant3	5′-CGAGGATCCCTAACCAGCGTAGTCTGGAACGTCGTATGGGTATCCACCTCCAGCATTCTCACGCCATTGAAATTTGG
C102f	5′-CCTTTTATCGTTGTACCAAAtgcAAATTTCAATGGCGTG
C102Sr	5′-CACGCCATTGAAATTTgcaTTTGGTACAACGATAAAAGG
C11DGSen	5′-CAAATTCGTAGTGCAGGTGAACCTATGAGTACCTTTT
C11DGAnt	5′-AAAAGGTACTCATAGGTTCACCTGCACTACGAATTTG
Hssen1	5′-AACAACTCGAGATGCTGCTGTTCTGCCCCGGCT; XhoI
Hsan1	5′-AACAGGATCCCTAATCCCTCCAGCGGTGTCCAC; BamHI
Ala102f	5′-CCTTTTATCGTTGTACCAAAgcgAAATTTCAATGGCGTG
Ala102r	5′-CACGCCATTGAAATTTcgcTTTGGTACAACGATAAAAGG
Asn102f	5′-CCTTTTATCGTTGTACCAAAaatAAATTTCAATGGCGTG
Asn102r	5′-CACGCCATTGAAATTTattTTTGGTACAACGATAAAAGG
Arg102f	5′-CCTTTTATCGTTGTACCAAAagaAAATTTCAATGGCGTG
Arg102r	5′-CACGCCATTGAAATTTtctTTTGGTACAACGATAAAAGG
His102f	5′-CCTTTTATCGTTGTACCAAAcacAAATTTCAATGGCGTG
His102r	5′-CACGCCATTGAAATTTgtgTTTGGTACAACGATAAAAGG
Ile102f	5′-CCTTTTATCGTTGTACCAAAattAAATTTCAATGGCGTG
Ile102r	5′-CACGCCATTGAAATTTaatTTTGGTACAACGATAAAAGG
Tyr102f	5′-CCTTTTATCGTTGTACCAAAtatAAATTTCAATGGCGTG
Tyr102r	5′-CACGCCATTGAAATTTataTTTGGTACAACGATAAAAGG
Asp102f	5′-CCTTTTATCGTTGTACCAAAgacAAATTTCAATGGCGTG
Asp102r	5′-CACGCCATTGAAATTTgtcTTTGGTACAACGATAAAAGG
Glu102f	5′-CCTTTTATCGTTGTACCAAAgagAAATTTCAATGGCGTG
Glu102r	5′-CACGCCATTGAAATTTctcTTTGGTACAACGATAAAAGG
Gly102f	5′-CCTTTTATCGTTGTACCAAAggaAAATTTCAATGGCGTG
Gly102r	5′-CACGCCATTGAAATTTtccTTTGGTACAACGATAAAAGG
Petc11s	5′-GAACATATGCAGTTTTGTCCTACTTGTGGAAA; NdeI
Petc11a	5′-GAAGGATCCCTAATTCTCACGCCATTGAAATTTGC; BamHI
R107Csen	5′-CAAATGCAAATTTCAATGGTGTGAGAATTAGGGATCC
R107Crev	5′-GGATCCCTAATTCTCACACCATTGAAATTTGCATTTG
RACE 1	5′-GTCCGAGTGGTTAAGGAGTTAGACTTC
Reverse	5′-ACTGGAATTCGCGGTCTTTTA
3′ Adapter	5′-rArArArAdGdAdCdCdGdCdGdAdAdTdTdCdCdAdG-invdT

^aRestriction sites are underlined. Lowercase letters indicate mutated codons.