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. 2017 May 2;2017:3160360. doi: 10.1155/2017/3160360

Figure 2.

Figure 2

Role of Ras signaling in HIV-1 Tat-induced disruption of ZO-1. Before HIV-1 Tat exposure, HBEC-5i cells were pretreated with 5 μmol/L FTS for 3 h. FTS remained in the culture medium during Tat exposure. The time of Tat exposure was 24 h for western blotting (a) and 12 h for RT-PCR (b). HIV-1 Tat exposure induced decreased ZO-1 protein and mRNA levels in HBEC-5i cells. Following cotreatment with FTS and HIV-1 Tat, ZO-1 protein and mRNA levels were significantly increased compared with those in the HIV-1 Tat group. Data are shown as means ± standard error of the mean (n = 3 for (a), n = 5 for (b)). ∗∗p < 0.01 versus control; ##p < 0.01 versus HIV-1 Tat.