FIG. 5.

Passage though S phase is not required for changes in gene expression that occur upon Asf1p degradation. Cultures of strains SRH015 (WT) and SRH014 (Asf1-Pest) were arrested in G₁ as in Fig. 3 and then released into glucose-containing medium with or without 200 mM HU. Samples were taken before release from G₁ (t = 0), 45 min after release into HU or 45 min after release into medium containing no HU. (A) Flow cytometry analysis of DNA content by propidium iodide staining. (B) RT-PCR analysis of SRL3 gene expression. SWD2 was included as an internal control. (C) RT-PCR analysis of HYR1 gene expression. CDC42 was included as an internal control. (D) Quantitation of RT-PCR analysis of SRL3 for four independent experiments, as described in Fig. 4B. (E) Quantitation of RT-PCR analysis of HYR1 as described above.