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. 2005 Jan;25(2):699–705. doi: 10.1128/MCB.25.2.699-705.2005

FIG. 3.

FIG. 3.

Analysis of ECM deposition in the tendon. (A) Similar signal intensities were observed by immunostaining for collagen types I (col1α2), matrilin-2 (matn2), and decorin in P14 Achilles tendon. Collagen type III (col3α1) signals were reduced in Tnmd-deficient tendon. Bar, 100 μm. (B) Coomassie blue staining of P14 tail tendon extracts showed similar intensities of the collagen α1(I) band. No differences in signal intensities were found by probing for collagen types III (col3α1, 130 kDa) and VI (col6α3, 180 to 200 kDa), matrilin-2 (matn2, 120 to 150 kDa), and decorin (90 to 120 kDa). (C) Immunostaining for the different α chains of collagen VI revealed similar signal intensities for the collagen VI α1 chain (col6α2) and the α2 chain (col6α2), whereas decreased signal intensity was obtained for the α3 chain (col6α3). Bar, 100 μm. (D) Tnmd and collagen VI α3 showed similar distribution patterns with a predominant pericellular localization. Bar, 10 μm.