Table 2. Number and types of cellular abnormalities observed in root meristem cells of Allium cepa treated with water and synthetic grape and plum flavourings.
(V/m)/(mL/kg) | t(exposure)/h | Colchicine metaphase | Anaphase bridge | Telophase bridge | Micronucleus | Binucleate cell |
N(TCA) |
---|---|---|---|---|---|---|---|
Grape flavouring | |||||||
control | 0 | 0 | 1 | 0 | 0 | 1a | |
3.5 | 24 | 1 | 0 | 0 | 0 | 0 | 1a |
48 | 22 | 49 | 13 | 89 | 0 | 173b | |
control | 0 | 0 | 1 | 0 | 0 | 1a | |
7.0 | 24 | 9 | 72 | 22 | 111 | 3 | 217b |
48 | 0 | 37 | 59 | 98 | 1 | 195b | |
control | 0 | 1 | 0 | 0 | 0 | 1a | |
14.0 | 24 | 0 | 28 | 33 | 171 | 2 | 234b |
48 | 18 | 20 | 49 | 108 | 0 | 197b | |
Plum flavouring | |||||||
control | 1 | 0 | 0 | 0 | 0 | 1a | |
3.5 | 24 | 7 | 11 | 8 | 47 | 9 | 73b |
48 | 4 | 13 | 9 | 35 | 0 | 61c | |
control | 0 | 1 | 0 | 0 | 0 | 1a | |
7.0 | 24 | 9 | 13 | 13 | 59 | 0 | 94b |
48 | 9 | 17 | 9 | 62 | 0 | 97b | |
control | 1 | 0 | 0 | 0 | 0 | 1a | |
14.0 | 24 | 13 | 18 | 7 | 55 | 0 | 93b |
48 | 5 | 27 | 17 | 39 | 1 | 88b |
V=volume of the flavouring solution, m=mass (on average 0.2 kg) of onion bulb used, N(TCA)=number of total cellular alterations. Within the same treatment, TCA values followed by different letters are significantly different 5% by χ2 test