Table 2. HS-SPME-GC/MS profiles of volatile aroma compounds in Malvazija istarska, Chardonnay and Muscat yellow monovarietal wines produced in 2013 and 2014.
| Volatile aroma compound | LRI | ID | RM | FM | 1-way ANOVA | 2-way ANOVA | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2013 | 2014 | ||||||||||||||
| Malvazija istarska | Chardonnay | Muscat yellow | Malvazija istarska | Chardonnay | Muscat yellow | V | Y | I | |||||||
| γ(volatile compound)/(µg/L) or *(mg/L) | |||||||||||||||
| Terpenes | |||||||||||||||
| Epoxylinalool | 1111 | MS, LRI | 802 | 721 | (0.6±0.4)b | (0.1±0.1)c | (7.1±2.0)a | (0.2±0.2)b | (0.1±0.2)b | (3.2±0.8)a | ** | ** | ** | ||
| β-Pinene | 1145 | MS, LRI | 863 | 863 | (2.8±1.1)b | (0.1±0.1)c | (41.9±17.0)a | (2.9±1.8)b | (0.7±0.9)b | (44.7±14.3)a | ** | n.s. | n.s. | ||
| Limonene | 1196 | MS, LRI | 854 | 831 | (2.5±0.1)b | (1.9±0.5)b | (27.3±4.5)a | (1.9±0.9)b | (1.2±0.7)b | (21.5±4.0)a | ** | ** | n.s. | ||
| β-Phellandrene | 1203 | MS, LRI | 865 | 844 | n.d. | n.d. | 1.9±0.85 | n.d. | n.d. | 1.7±0.2 | ** | n.s. | n.s. | ||
| trans-β-Ocimene | 1253 | MS, LRI | 812 | 793 | n.d. | n.d. | 11.7±4.43 | (0.5±0.9)b | (0.1±0.2)b | (13.4±3.7)a | ** | n.s. | n.s. | ||
| α-Terpinolene | 1281 | MS, LRI | 894 | 864 | (0.1±0.2)b | n.d. | (6.0±1.6)a | (0.3± 0.5)b | n.d. | (6.5±3.0)a | ** | n.s. | n.s. | ||
| Monoterpene (n.i.) | 1322 | MS | n.d. | n.d. | 14.2±5.4 | n.d. | n.d. | 10.4±3.9 | ** | n.s. | n.s. | ||||
| 1,3,5,5-Tetramethyl-1,3-cyclohexadiene | 1370 | MS | 865 | 801 | n.d. | n.d. | 3.4±1.3 | n.d. | n.d. | 3.0±0.9 | ** | n.s. | n.s. | ||
| trans-Linalool furan oxide | 1436 | MS, LRI | 873 | 790 | n.d. | n.d. | 19.3±5.4 | n.d. | n.d. | 10.8±2.2 | ** | ** | ** | ||
| p-Menth-1-en-8-ol | 1446 | MS | 807 | 734 | n.d. | n.d. | 16.8±3.0 | n.d. | n.d. | 7.4±3.2 | ** | ** | ** | ||
| 6,10-Dihydromyrcenol | 1457 | MS, LRI | 820 | 762 | 0.8±0.6 | 1.2±0.67 | 0.8±0.1 | 1.3±0.8 | 0.9±0.6 | 0.6±0.3 | n.s. | n.s. | n.s. | ||
| Nerol oxide | 1459 | MS, LRI | 828 | 795 | (2.2±1.1)b | (0.5±0.4)c | (29.1±8.1)a | (1.2±0.9)b | (0.6±0.8)b | (11.7±3.7)a | ** | ** | ** | ||
| cis-Linalool furan oxide | 1464 | MS, LRI | 888 | 804 | n.d. | (0.2±0.4)b | (9.2±3.3)a | (0.4±0.5)b | n.d. | (3.8±1.1)a | ** | ** | ** | ||
| Geranyl ethyl ether 1 | 1499 | MS, LRI | 828 | 795 | n.d. | n.d. | 13.0±4.5 | n.d. | n.d. | 6.2±2.7 | ** | ** | ** | ||
| Monoterpene (n.i.) | 1502 | MS | (0.2±0.3)b | n.d. | (4.5±3.8)a | (0.1±0.1)b | (0.0±0.0)b | (1.5±0.8)a | ** | n.s. | n.s. | ||||
| Linalool | 1542 | S, MS, LRI | 843 | 835 | (54.1±21.3)b | (20.4±11.3)c | (670.5±259.1)a | (33.6±19.0)b | (18.9±7.8)b | (723.3±131.2)a | ** | n.s. | n.s. | ||
| Isobornyl acetate | 1587 | MS, LRI | 886 | 851 | 0.3±0.1 | 0.3±0.1 | 0.3±0.1 | 0.3±0.1 | 0.2±0.1 | 0.3±0.1 | n.s. | n.s. | n.s. | ||
| Hotrienol | 1601 | MS, LRI | 831 | 788 | (4.7±2.1)b | (1.0±0.6)c | (90.6±25.7)a | (2.5±2.4)b | (1.2±1.0)b | (55.3±11.5)a | ** | ** | ** | ||
| Menthol | 1628 | MS, LRI | 914 | 814 | 3.4±1.3 | 2.4±1.3 | 1.7±0.8 | (3.2±1.2)a | (2.5±1.6)a | (1.2±0.8)b | ** | n.s. | n.s. | ||
| α-Terpineol | 1684 | S, MS, LRI | 850 | 830 | (23.7±9.2)b | (7.9±4.4)c | (191.2±38.2)a | (10.8±7.3)b | (8.1±5.2)b | (124.5±38.6)a | ** | ** | ** | ||
| α-Terpinenyl acetate | 1688 | MS, LRI | 871 | 780 | (0.9±0.4)b | (0.6±0.1)b | (3.5±0.5)a | (0.7±0.3)b | (0.6±0.4)b | (2.4±0.6)a | ** | ** | ** | ||
| Geranyl acetate | 1749 | MS, LRI | 878 | 816 | n.d. | n.d. | n.d. | n.d. | n.d. | 3.5±2.1 | ** | ** | ** | ||
| trans-Linalool pyran oxide | 1752 | MS, LRI | 809 | 800 | (0.1±0.2)b | n.d. | (10.2±3.4)a | (0.6±0.8)b | n.d. | (5.5±2.1)a | ** | ** | ** | ||
| Citronellol | 1758 | S, MS, LRI | 864 | 778 | (4.0±0.8)b | (3.5±0.7)b | (23.2±13.2)a | (3.7±1.6)b | (2.9±0.5)b | (31.9±14.4)a | ** | n.s. | n.s. | ||
| Nerol | 1791 | S, MS, LRI | 929 | 807 | (5.7±2.8)b | (0.9±1.1)c | (70.7±24.4)a | (4.0±2.0)b | (0.7±0.5)c | (76.3±15.8)a | ** | n.s. | n.s. | ||
| Geraniol | 1838 | S, MS, LRI | 902 | 811 | (23.2±5.2)b | (14.0±3.2)c | (196.5±45.3)a | (16.4±8.2)b | (12.747±3.4)b | (170.0±45.7)a | ** | n.s. | n.s. | ||
| Geranyl acetone | 1845 | MS, LRI | 885 | 811 | (0.8±0.5)b | (1.1±0.4)b | (3.2±1.2)a | (1.2±0.9)b | (1.1±1.0)b | (2.9±1.4)a | ** | n.s. | n.s. | ||
| trans-Nerolidol | 2031 | MS, LRI | 874 | 804 | 1.4±0.3 | 1.8±0.8 | 2.0±1.0 | 0.89±0.3 | 1.0±0.3 | 1.3±0.3 | n.s. | ** | n.s. | ||
| Geranic acid | 2319 | MS, LRI | 838 | 749 | n.d. | n.d. | 4.7±1.8 | n.d. | n.d. | 7.0±2.2 | ** | n.s. | n.s. | ||
| Farnesol | 2341 | MS, LRI | 907 | 799 | 0.3±0.2 | 0.6±0.3 | 0.7±0.5 | (0.4±0.1)b | (0.6±0.1)ab | (0.7±0.2)a | n.s. | n.s. | n.s. | ||
| Total terpenes | (130.8±39.6)b | (58.0±21.8)c | (1474.7±384.5)a | (86.7±43.6)b | (54.1±18.9)b | (1352.3±197.3)a | ** | n.s. | n.s. | ||||||
| C13 norisoprenoids | |||||||||||||||
| β-damascenone | 1809 | S, MS, LRI | 853 | 830 | 1.1±0.3 | 1.4±0.3 | 1.0±0.5 | 1.3±0.4 | 1.3±0.3 | 1.2±0.6 | n.s. | n.s. | n.s. | ||
| Norisoprenoid (n.i.) | 2119 | MS | 0.9±0.4 | 1.3±0.4 | 0.8±0.5 | 1.0±0.4 | 0.8±0.1 | 1.8±1.7 | n.s. | n.s. | n.s. | ||||
| Total C13 norisoprenoids | (1.9±0.7)ab | (2.7±0.5)a | (1.8±0.6)b | 2.3±0.7 | 2.1±0.3 | 3.0±1.2 | n.s. | n.s. | n.s. | ||||||
| C6 compounds | |||||||||||||||
| Hexanal | 1080 | MS, LRI | 889 | 823 | 1.7±0.4 | 2.4±0.7 | 4.2±4.1 | 2.4±1.2 | 3.1±1.3 | 3.3±1.7 | n.s. | n.s. | n.s. | ||
| cis-3-Hexen-1-yl acetate | 1316 | MS, LRI | 826 | 803 | (116.3±65.9)b | (152.3±43.2)ab | (277.4±160.5)a | 78.8±48.3 | 78.7±17.5 | 210.3±154.0 | ** | n.s. | n.s. | ||
| 1-Hexanol* | 1356 | S, MS, LRI | 876 | 817 | 1.3±0.4 | 1.1±0.1 | 1.1±0.2 | (1.4±0.3)a | (1.3±0.3)ab | (1.0±0.1)b | ** | n.s. | n.s. | ||
| trans-3-Hexen-1-ol | 1361 | MS, LRI | 861 | 839 | (41.6±12.9)a | (32.1±13.8)ab | (15.5±4.8)b | 33.9±14.1 | 31.1±11.7 | 27.8±15.7 | n.s. | n.s. | n.s. | ||
| cis-3-Hexen-1-ol | 1379 | MS, LRI | 871 | 844 | (51.6±13.2)b | (38.4±2.4)b | (141.4±52.0)a | (34.3±10.0)b | (32.0±10.4)b | (87.8±33.6)a | ** | ** | n.s. | ||
| Total C6 compounds* | 1.5±0.4 | 1.3±0.2 | 1.5±0.2 | 1.6±0.3 | 1.4±0.3 | 1.3±0.3 | n.s. | n.s. | n.s. | ||||||
| Alcohols | |||||||||||||||
| 2-Ethyl-1-hexanol | 1485 | MS, LRI | 851 | 835 | 11.4±3.3 | 9.3±3.9 | 17.8±15.2 | 9.1±3.7 | 11.8±7.1 | 7.3±2.1 | n.s. | n.s. | n.s. | ||
| 1-Octanol | 1552 | MS, LRI | 887 | 841 | (19.9±2.3)a | (16.9±4.7)ab | (14.8±2.3)b | 16.5±2.5 | 16.7±1.5 | 15.0±3.8 | n.s. | n.s. | n.s. | ||
| 2-Phenylethanol* | 1893 | S, MS, LRI | 956 | 956 | 21.7±5.6 | 16.1±2.2 | 16.5±6.5 | 13.5±2.6 | 17.1±5.1 | 13.6±1.3 | n.s. | n.s. | n.s. | ||
| Total alcohols* | 21.7±5.6 | 16.1±2.2 | 16.6±6.5 | 13.5±2.6 | 17.1±5.1 | 13.6±1.4 | n.s. | n.s. | n.s. | ||||||
| Fatty acids | |||||||||||||||
| Hexanoic acid* | 1830 | S, MS, LRI | 813 | 738 | (12.0±1.7)a | (12.9±2.1)a | (8.5±2.7)b | 8.6±2.1 | 9.0±3.1 | 9.7±2.7 | n.s. | n.s. | n.s. | ||
| Octanoic acid* | 2043 | S, MS, LRI | 889 | 759 | 11.6±1.2 | 11.3±3.2 | 9.9±3.6 | 7.4±2.4 | 7.9±2.5 | 12.0±4.8 | n.s. | n.s. | n.s. | ||
| Nonanoic acid* | 2135 | MS, LRI | 831 | 802 | (0.3±0.1)ab | (0.8±0.6)a | (0.2±0.1)b | (1.1±0.3)a | (1.4±0.7)a | (0.3±0.1)b | ** | ** | n.s. | ||
| Decanoic acid* | 2257 | S, MS, LRI | 820 | 805 | 2.1±0.7 | 1.7±0.7 | 3.2±1.5 | (0.8±0.3)b | (1.0±0.3)b | (4.0±2.1)a | ** | n.s. | n.s. | ||
| Dodecanoic acid | 2467 | MS, LRI | 869 | 865 | 125.3±42.0 | 101.7±40.6 | 127.7±68.2 | 64.2±13.0 | 109.4±27.8 | 126.7±79.4 | n.s. | n.s. | n.s. | ||
| Total fatty acids* | 26.1±3.1 | 26.7±5.9 | 21.9±7.7 | 18.0±4.4 | 19.5±5.5 | 26.1±9.4 | n.s. | n.s. | n.s. | ||||||
| Ethyl esters | |||||||||||||||
| Ethyl propionate | <1000 | MS, LRI | 835 | 760 | 1.6±0.2 | 2.0±0.6 | 1.7±0.3 | 1.5±0.3 | 1.5±0.3 | 1.2±0.2 | n.s. | ** | n.s. | ||
| Ethyl isobutyrate | <1000 | MS, LRI | 811 | 721 | (33.6±9.0)a | (17.1±5.6)b | (24.2±4.7)ab | 16.8±9.6 | 18.6±8.2 | 17.2±6.7 | n.s. | ** | n.s. | ||
| Ethyl butyrate* | 1030 | S, MS, LRI | 859 | 766 | 0.5±0.1 | 0.5±0.1 | 0.5±0.1 | 0.45±0.07 | 0.5±0.1 | 0.5±0.1 | n.s. | n.s. | n.s. | ||
| Ethyl 2-methylbutyrate | 1049 | S, MS, LRI | 851 | 800 | (8.0±2.8)a | (4.0±1.5)b | (3.4±1.0)b | 3.3±1.0 | 3.7±1.6 | 2.0±0.9 | ** | * | ** | ||
| Ethyl 3-methylbutyrate | 1065 | S, MS, LRI | 800 | 722 | (19.4±6.1)a | (9.3±3.6)b | (8.8±1.0)b | 9.2±1.9 | 8.2±4.8 | 5.2±3.0 | ** | ** | n.s. | ||
| Ethyl 2-butenoate | 1161 | MS, LRI | 852 | 844 | 2.0±1.0 | 2.1±0.3 | 1.5±0.7 | 2.8±1.1 | 2.2±0.5 | 2.2±1.1 | n.s. | n.s. | n.s. | ||
| Ethyl hexanoate* | 1236 | S, MS, LRI | 868 | 826 | 0.6±0.1 | 0.8±0.1 | 0.7±0.2 | (0.4±0.2)b | (0.5±0.2)b | (0.8±0.1)a | ** | n.s. | ** | ||
| Ethyl heptanoate | 1332 | MS, LRI | 849 | 804 | (11.2±3.5)b | (20.9±7.7)a | (8.4±3.3)b | (27.2±8.7)ab | (32.9±8.3)a | (16.5±7.2)b | ** | ** | n.s. | ||
| Ethyl octanoate* | 1435 | S, MS, LRI | 862 | 784 | (1.1±0.3)b | (1.8±0.4)a | (1.3±0.9)a | (1.0±0.3)b | (1.3±0.6)ab | (1.8±0.4)a | ** | n.s. | n.s. | ||
| Ethyl nonanoate | 1534 | MS, LRI | 820 | 786 | (18.3±8.1)ab | (34.2±17.6)a | (10.4±5.2)b | (32.4±9.5)ab | (46.5±19.5)a | (16.1±2.6)b | ** | ** | n.s. | ||
| Ethyl decanoate* | 1637 | S, MS, LRI | 830 | 770 | 0.39±0.05 | 0.4±0.1 | 0.7±0.4 | (0.5±0.2)ab | (0.36±0.08)b | (0.6±0.2)a | ** | n.s. | n.s. | ||
| Ethyl dodecanoate | 1842 | MS, LRI | 769 | 769 | 9.9±0.7 | 12.0±2.5 | 12.1±6.8 | 9.4±1.8 | 12.8±1.5 | 12.3±3.3 | n.s. | n.s. | n.s. | ||
| Ethyl tetradecanoate | 2045 | MS, LRI | 803 | 800 | 7.4±0.7 | 8.0±1.5 | 6.0±2.4 | 5.9±1.8 | 6.91±1.5 | 7.9±2.5 | n.s. | n.s. | n.s. | ||
| Total ethyl esters* | 2.6±0.5 | 3.8±0.4 | 3.2±1.5 | 2.5±0.6 | 2.8±0.9 | 3.3±1.2 | n.s. | n.s. | n.s. | ||||||
| Acetate esters | |||||||||||||||
| Methyl acetate | <1000 | MS, LRI | 888 | 861 | 2.5±0.7 | 2.6±0.7 | 2.4±0.8 | 3.7±1.0 | 3.0±1.0 | 3.6±2.1 | n.s. | n.s. | n.s. | ||
| Isobutyl acetate | 1009 | S, MS, LRI | 842 | 734 | (58.0±18.2)b | (111.0±13.7)a | (85.0±28.5)ab | 97.3±38.4 | 132.4±17.2 | 133.0±17.7 | ** | ** | n.s. | ||
| Butyl acetate | 1072 | MS, LRI | 868 | 807 | (3.4±1.9)b | (7.3±1.8)a | (4.6±3.1)ab | (4.9±1.6)b | (9.6±4.4)a | (4.5±2.4)b | ** | n.s. | n.s. | ||
| Isoamyl acetate* | 1120 | S, MS, LRI | 885 | 839 | 3.2±1.2 | 5.4±0.8 | 4.3±2.5 | 4.0±1.6 | 4.6±0.7 | 5.2±2.3 | n.s. | n.s. | n.s. | ||
| Hexyl acetate* | 1272 | S, MS, LRI | 905 | 822 | 0.15±0.04 | 0.23±0.06 | 0.2±0.1 | 0.18±0.08 | 0.17±0.04 | 0.3±0.1 | n.s. | n.s. | n.s. | ||
| 2-Phenylethyl acetate* | 1803 | S, MS, LRI | 856 | 856 | (2.9±0.8)b | (4.6±0.5)a | (3.0±0.9)b | 2.1±0.5 | 3.6±0.7 | 3.4±1.5 | ** | n.s. | n.s. | ||
| Total acetate esters * | (6.3±1.8)b | (10.7±1.3)a | (7.6±3.3)ab | 6.4±1.8 | 8.4±0.6 | 9.0±3.9 | n.s. | n.s. | n.s. | ||||||
| Other esters | |||||||||||||||
| Methyl hexanoate | 1192 | MS, LRI | 876 | 844 | 1.2±0.4 | 1.3±0.6 | 0.9±0.3 | 1.1±0.4 | 0.7±0.3 | 1.2±0.4 | n.s. | n.s. | n.s. | ||
| Isoamyl formate | 1196 | MS | 819 | 751 | 0.7±1.0 | 0.9±1.4 | 0.4±0.2 | 0.2±0.1 | 0.7±0.6 | 0.4±0.1 | n.s. | n.s. | n.s. | ||
| Methyl octanoate | 1387 | MS, LRI | 871 | 823 | 18.6±2.2 | 18.7±1.3 | 15.7±2.8 | 17.4±2.6 | 17.2±3.1 | 20.8±6.2 | n.s. | n.s. | n.s. | ||
| Isoamyl hexanoate | 1457 | MS, LRI | 835 | 835 | (0.7±0.3)b | (1.5±0.4)ab | (2.0±0.8)a | 1.3±0.6 | 1.4±0.4 | 1.6±0.4 | ** | n.s. | n.s. | ||
| Isoamyl heptanoate | 1560 | MS | 822 | 798 | 0.9±0.8 | 1.5±1.3 | 1.0±0.7 | 1.2±0.6 | 1.2±0.5 | 0.6±0.5 | n.s. | n.s. | n.s. | ||
| Isoamyl octanoate | 1655 | MS, LRI | 800 | 722 | 6.4±2.1 | 6.2±1.6 | 4.8±1.9 | 5.7±1.2 | 7.3±1.0 | 8.3±2.6 | n.s. | n.s. | n.s. | ||
| Diethyl succinate* | 1667 | S, MS, LRI | 859 | 827 | (2.2±0.9)a | (1.7±0.8)ab | (0.9±0.3)b | 1.1±0.6 | 1.20±0.1 | 0.9±1.1 | n.s. | n.s. | n.s. | ||
| Ester (n.i.) | 1710 | MS | (3.4±1.0)b | (4.9±0.4)a | (6.1±1.2)a | (2.5±0.4)b | (3.4±1.0)ab | (5.0±1.8)a | ** | ** | n.s. | ||||
| Methyl salicylate | 1759 | MS, LRI | 887 | 852 | (0.9±0.5)a | (0.24±0.07)b | (0.09±0.03)b | 0.5±0.1 | 0.5±0.3 | 0.4±0.1 | ** | n.s. | ** | ||
| Isoamyl decanoate | 1862 | MS, LRI | 800 | 748 | 1.0±0.2 | 0.8±0.4 | 0.7±0.4 | (3.6±3.1)a | (0.6±0.2)b | (0.9±0.4)ab | ** | n.s. | n.s. | ||
| Ethyl cinnamate | 2111 | S, MS, LRI | 848 | 790 | (1.4±0.3)b | (3.2±1.0)a | n.d. | (1.0±0.3)b | (2.3±0.5)a | (0.8±1.0)b | ** | n.s. | ** | ||
| Hexyl salicylate | 2203 | MS, LRI | 801 | 771 | 1.0±0.1 | 1.0±0.2 | 1.3±0.3 | 0.8±0.3 | 0.7±0.2 | 1.0±0.2 | ** | ** | n.s. | ||
| Total other esters* | (2.2±1.0)a | (1.8±0.8)ab | (0.9±0.3)b | 1.2±0.6 | 1.24 ± 0.09 | 0.9±1.1 | n.s. | n.s. | n.s. | ||||||
| Miscellaneous | |||||||||||||||
| 2-Heptanone | 1182 | MS, LRI | 875 | 800 | 1.5±0.7 | 1.2±1.1 | 1.3±1.2 | (0.9±0.4)ab | (0.8±0.2)b | (1.4±0.3)a | n.s. | n.s. | n.s. | ||
| Benzaldehyde | 1508 | S, MS, LRI | 864 | 864 | (4.2±1.1)b | (9.5±1.7)a | (2.5±0.7)c | 10.1±6.8 | 12.7±4.9 | 10.8±4.8 | n.s. | ** | n.s. | ||
| Ethyl benzeneacetate | 1773 | MS, LRI | 914 | 858 | 1.2±0.4 | 1.3±0.5 | 1.4±1.2 | 2.2±1.5 | 2.2±0.8 | 2.6±1.7 | n.s. | ** | n.s. | ||
| Dimethylbenzaldehyde isomer | 1790 | MS | 873 | 873 | (17.8±2.5)b | (24.9±4.1)a | (2.4±1.1)c | (7.4±5.4)b | (13.4±3.0)a | (2.8±2.0)b | ** | ** | ** | ||
| γ-Nonalactone | 2008 | MS, LRI | 839 | 763 | 0.9±0.5 | 0.7±0.2 | 1.1±0.5 | 1.5±0.9 | 1.0±0.2 | 2.4±1.3 | ** | ** | n.s. | ||
| 4’-Ethoxy-2’-hydroxyoctanophenone | 2090 | MS, LRI | 759 | 724 | 2.7±0.6 | 3.1±0.5 | 3.7±1.1 | (2.1±0.7)b | (1.8±0.6)b | (4.0±1.9)a | ** | n.s. | n.s. | ||
| 4-Vinylguaiacol | 2175 | MS, LRI | 903 | 903 | 7.2±1.5 | 6.3±6.6 | 8.1±4.0 | 5.1±2.3 | 6.8±2.6 | 8.1±3.8 | n.s. | n.s. | n.s. | ||
| 2-(Phenylmethylene)-octanal | 2356 | MS, LRI | 839 | 832 | 4.2±1.3 | 3.4±1.3 | 4.0±0.9 | (2.5±1.2)b | (2.1±0.8)b | (4.4±1.0)a | ** | n.s. | n.s. | ||
| 1,2-Benzenedimethanol | 2376 | MS | 853 | 822 | 2.5±1.3 | 5.9±6.1 | 2.0±0.8 | 1.6±0.7 | 5.4±2.5 | 5.9±4.8 | n.s. | n.s. | n.s. | ||
| Total miscellaneous | (42.5±5.1)b | (56.3±13.3)a | (26.9±5.2)c | 33.6±11.0 | 46.5±3.8 | 42.9±12.2 | ** | n.s. | ** | ||||||
LRI=linear retention index; ID=identification of compounds: S=retention time and mass spectrum consistent with that of the pure standard and with NIST05 mass spectra electronic library, MS=mass spectra consistent with those from NIST05 mass spectra electronic library, LRI=linear retention index consistent with that found in literature (39–44). The compounds for which pure standards were not available (without letter S in the ID column) were quantified semi-quantitatively, and their concentrations were expressed as equivalents of compounds with similar chemical structure assuming a response factor=1. The compounds with only MS symbol in the ID column were tentatively identified. RM=mass spectra reverse match number; FM=mass spectra forward match number; n.i.=not identified; n.d.=not detected. Different lowercase superscript letters in a row represent statistically significant differences between mean values at p<0.05 obtained by one-way ANOVA and Fisher’s least significant difference (LSD) test for each harvest year separately. Two-way ANOVA factors: V=variety, Y=harvest year, I=interaction (V×Y); **statistically significant effect at p<0.05 by 2-way ANOVA; n.s.=not significant