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. Author manuscript; available in PMC: 2018 Mar 27.
Published in final edited form as: Dev Cell. 2017 Mar 17;40(6):595–607.e4. doi: 10.1016/j.devcel.2017.02.017

Figure 3. Foxh1 is required for the early blastula recruitment of the co-repressor Tle.

Figure 3

A) A schematic diagram showing domains present in the Foxh1 protein. B) Heatmap of stage 8 Tle ChIP-seq signal centered on the stage 8 Foxh1 peaks. C) Peak calling identified 16,856 overlapping Foxh1 and Tle peaks. D) IP-western blotting reveals the significant reduction of Foxh1 protein at stage 6 (32-cells) after injection of a translation blocking morpholino oligonucleotide (MO) targeting foxh1 mRNA. E) Genome browser visualization of selected Tle/Foxh1 overlapping peaks near target genes cer1 (scaffold_1:102,363,840-102,373,840), gsc (scaffold_8:62,417,448-62,431,448), and nodal1 and nodal2 (scaffold_3:5,671,591-5,690,591). The boxed enhancers were analyzed using ChIP-qPCR in panel (F) below. Ep300 tracks show that many of these Foxh1/Tle-bound regions are active CRMs (see also Figure S5B). F) Stage 8 Tle ChIP-qPCR was performed in control and foxh1 MO-injected embryos. One representative experiment is shown with mean fold enrichment over the eef1a1 background region +/− SD.