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. Author manuscript; available in PMC: 2017 May 17.
Published in final edited form as: Dev Dyn. 2015 Nov 3;245(1):22–33. doi: 10.1002/dvdy.24355

Figure 3. Role of Kupffer’s vesicle size in LR patterning.

Figure 3

(A–D) Modulating Cftr activity was used to alter KV lumen size at 8 ss. The Cftr activating drug CFTact-09 increased KV size (B), whereas a loss-of-function mutation (C) or morpholino (MO) interference (D) reduced KV size relative to controls (A). Dashed circles represent approximate KV lumen boundaries. (E–H) Possible outcomes of RNA in situ hybridization analysis of the LR patterning marker spaw in lateral plate mesoderm (arrowhead) at 18 ss are normal left-sided (E), bilateral (F), reversed right-sided (G) or absent (H) expression. Dashed lines indicate the embryonic midline. L=left; R=right. (I–K) Quantification of KV area (I), cilia number (J) and cilia length (K) in embryos with altered Cftr function and controls. (L) Analysis of spaw expression in Cftr modulated embryos. Error bars represent one standard deviation. n=number of embryos analyzed. * indicates p < 0.05