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. 2017 May 16;5:56. doi: 10.1186/s40168-017-0273-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Location of I-1G6-GFP. Micrographs illustrating the preferential location of 1G6-GFP cells on biofilm (b) compared to root (r) at 3 hpi (a), 48 hpi (d, e), and 8 dpi (f). The colonization observed for I-1G6-GFP at 3 hpi (a) 8 dpi (f) can be compared to the lesser one observed for E. coli-GFP (b) and (g), respectively. c Histogram of the relative fluorescence intensity at 3 and 24 hpi for I-1G6-GFP and E.coli-GFP, measured as the ratio of the mean values at the surface of the biofilms and of roots. Bars: 200 μm in a, b, f, and g; 50 μm in d and e. The dotted lines delineate the interface between biofilm and root. In (e) dotted lines delineate an epidermal cell infected by I-1G6-GFP-and located beneath a biofilm