Table 2.

Upregulated protein spots identified as carbohydrate-active enzymes

Spot no.	Method	No. of peptides	Of which unique	Score (−10lgP)	Database entry by PEAKS DB and SPIDER	Name	Categorya
1	PEAKS DB SPIDER	8	2	216.7	Gi|489608295b	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		9	3	246.3
2	PEAKS DB SPIDER	8	2	162.1	Gi|489608295b	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		11	4	172.5
3	PEAKS DB SPIDER	5	2	109.7	Gi|489608295b Gi|740458809c	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		13	8	148.5
4	PEAKS DB SPIDER	4	2	108.6	Gi|489608295b Gi|740458809c	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		4	2	116.6
5	PEAKS DB SPIDER	1	1	91.99	Gi|489608295b	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		1	1	91.99
6	PEAKS DB SPIDER	7	5	126.8	Gi|489614249b	Cellulose 1,4-β-cellobiosidase	CAZy GH 9, NC, SP (1–27)
		8	3	133.8
7	PEAKS DB SPIDER	17	4	216.7	Gi|575088796c	Endo 1-4-β-xylanase	CAZy GH 11, NC, SP (1–29)
		34	14	246.3
8	PEAKS DB SPIDER	4	2	121.9	Gi|489614855b Gi|489609419c	Carbohydrate-binding protein Endo 1-4-β-xylanase precursor	CAZy GH 11, NC, SP (1–29)
		4	4	121.9
9	PEAKS DB SPIDER	5	3	97.4	Gi|489614249b Gi|575088796c	Cellulose 1,4-β-cellobiosidase Endo 1-4-β xylanase	CAZy GH 9, NC, SP (1–27) CAZy GH 11, NC, SP (1–29)
		6	3	106.1
10	PEAKS DB SPIDER	8	2	149.6	Gi|575088170c	Cellobiose phosphorylase	CAZy GH 11, TM, SP (1–27)
		9	3	152.6
11	PEAKS DB SPIDER	9	2	136.7	Gi|575088170c Gi|489613736b	Cellobiose phosphorylase Glycosyl transferase	CAZy GH 94, TM
		9	2	139.2
12	PEAKS DB SPIDER	2	1	74.76	Gi|740457862c	Glycosyl transferase	CAZy GH 94, TM
		2	1	74.76
13	PEAKS DB SPIDER	7	2	120.6	Gi|489616357b	β-Glucosidase	CAZy GH 1, TM (weak), NC
		9	2	124.2
14	PEAKS DB SPIDER	14	4	243.8	Gi|553726407b Gi|489608943b	Hypothetical Ig domain protein	Carbohydrate act. enzyme domain, NC, SP (1–26)
		22	10	260.9
15	PEAKS DB SPIDER	8	4	178.1	Gi|553726407b Gi|489608943b	Hypothetical Ig domain protein	Carbohydrate act. enzyme domain, NC, SP (1–26)
		12	7	193.2
16	PEAKS DB SPIDER	9	3	166.4	Gi|553726407b Gi|489608943b	Hypothetical Ig domain protein	Carbohydrate act. enzyme domain, NC, SP (1–26)
		15	5	184.8
17	PEAKS DB SPIDER	4	4	155.9	Gi:489613676b	Hypothetical Ig domain protein	Carbohydrate act. enzyme domain, NC, SP (1–26)
		4	4	155.9

^aIn CAZy database; GH glycoside hydrolase family no., NC non-cellulosomal, SP signal peptide, signal peptide sequence length in brackets, TM transmembrane

^b Ruminiclostridium thermocellum

^c Clostridium straminisolvens