Table A1. GenBank records analyzed for the standard COI barcode segment of adult Danaus plexippus, with haplotype designations used in the present study
| Country | GenBank accession no. | Voucher code | Date collected | COI haplotype | Base pairs (bp) |
|---|---|---|---|---|---|
| Costa Ricaa | GU333882 | 01–SRNP–25029 | 17 Dec 2001 | 2 | 574 |
| GU333883 | 00–SRNP–20751 | 20 Nov 2000 | 2 | 574 | |
| GU333885 | 98–SRNP–7209 | 19 Aug 1998 | 2 | 574 | |
| GU333886 | 98–SRNP–7254 | 24 Aug 1998 | 2 | 605 | |
| GU333887 | 00–SRNP–353 | 25 Feb 2000 | 5 | 591 | |
| GU333888 | 98–SRNP–7210 | 20 Aug 1998 | 1 | 572 | |
| GU333889 | 00–SRNP–353 | 16 Feb 2000 | 5 | 548 | |
| GU651737 | 09–SRNP–495 | 25 Feb 2009 | 2 | 658 | |
| GU666763 | 08–SRNP–70857 | 07 Jul 2008 | 2 | 658 | |
| GU666764 | 08–SRNP–31170 | 26 May 2008 | 2 | 658 | |
| GU666765 | 08–SRNP–70296 | 19 May 2008 | 2 | 658 | |
| GU666766 | 08–SRNP–70297 | 21 May 2008 | 2 | 658 | |
| GU666769 | 08–SRNP–72358 | 24 Sep 2008 | 2 | 658 | |
| GU666770 | 08–SRNP–12361 | 19 Aug 2008 | 2 | 658 | |
| HM884512 | 10–SRNP–103587 | 02 Apr 2010 | 2 | 658 | |
| HM905228 | 10–SRNP–103418 | 27 Mar 2010 | 1 | 658 | |
| HM905373 | 10–SRNP–103569 | 01 Apr 2010 | 1 | 658 | |
| HM905374 | 10–SRNP–103570 | 01 Apr 2010 | 2 | 658 | |
| JQ529767 | 10–SRNP–67640 | 05 Jul 2010 | 5 | 658 | |
| JQ529768 | 10–SRNP–67641 | 06 Jul 2010 | 5 | 658 | |
| JQ529799 | 10–SRNP–67008 | 27 Jan 2010 | 2 | 658 | |
| JQ543430 | 06–SRNP–3790 | 20 May 2006 | 1 | 656 | |
| JQ548240 | 05–SRNP–43782 | 05 Jan 2006 | 2 | 658 | |
| JQ536356 | 07–SRNP–45296 | 09 Jun 2007 | 2 | 658 | |
| JQ537098 | 07–SRNP–65886 | 15 Nov 2007 | 2 | 657 | |
| JQ553760 | 08–SRNP–108642 | 28 Nov 2008 | 2 | 658 | |
| Yucatan Peninsula, Mexico | GU659705 | MAL–02630 | 04 Jul 2002 | 1 | 588 |
| GU659706 | MAL–02631 | 05 Jul 2002 | 2 | 644 | |
| GU659707b | MAL–02633 | 24 May 1999 | 2 | 621 | |
| GU659709 | MAL–02626 | 08 Sep 2006 | 2 | 658 | |
| GU659710 | MAL–02627 | 07 Nov 2003 | 1 | 658 | |
| GU659711 | MAL–02628 | 11 Mar 2004 | 6 | 658 | |
| HM890805 | MAL–02629 | 25 Nov 2004 | 2 | 549 | |
| Canada (Ontario) | KM550302 | PPBP-1165 | 12 Jun 2008 | 1 | 658 |
| Australia | KF398175 | 11ANIC-06932 | 02 Feb 1968 | 1 | 658 |
| KF402604 | 11ANIC-06933 | 01 Dec 1984 | 1 | 658 | |
| KF403674 | 11ANIC-06931 | 23 Mar 1996 | 1 | 658 | |
| Spain | GU675768 | SMcoll.08-J600 | 22 Jun 2008 | 1 | 658 |
| JN266597 | RVcoll.130209KL61 | 01 Dec 2001 | 1 | 658 | |
| KP870908 | RVcoll. 07-C577 | 20 Jul 2005 | 1 | 658 | |
| KP871034 | RVcoll. 07-C576 | 20 Jul 2005 | 1 | 658 |
All sequences, except the one from Canada, are from nonmigratory populations.
Most adult monarchs from Costa Rica were obtained from larvae reared in the laboratory on Asclepias curassavica L. (Apocynaceae) (Janzen and Hallwachs 2009).
Examination of the original electropherogram revealed 3 sites in the 621 bp segment (sites no. 88, 187, and 190) that contained reading errors in the output file which were corrected.