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. 2017 May 16;18:7. doi: 10.1186/s12858-017-0084-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PNK/CPDase interacts with E. coli RNA. a Superdex 200 SEC profile of ScTrl1 PNK/CPDase (continuous lines) and BfPNK/CPDase (dashed lines). The peaks close to the void volume with A₂₆₀ (green) higher than A₂₈₀ (blue) indicate the presence of a nucleic acid. V_O: column void volume. b Agarose gels of BfPNK/CPDase and ScTrl1 PNK/CPDase. L: DNA ladder, 1: The protein-nucleic acid complex eluted near the void volume from SEC, 2: DNase-treated protein-nucleic acid complex, 3: RNase-treated protein-nucleic acid complex. RNase degrades the nucleic acid, confirming that the co-purified molecule is, in fact, RNA