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. 2017 May 16;10:239. doi: 10.1186/s13071-017-2181-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — A possible diagnostic approach based on qPCR and HRM analysis. The qPCR-ML followed by HRM analysis is performed. In the case of lack of amplification, or if HRM results are inconclusive, the qPCR-ama can also be performed. The evaluation of C_q values for both assays will allow discriminating between L. (L.) infantum and L. (L.) amazonensis