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Tm comparison of L. (L.) amazonensis and L. (L.) infantum amplicons showing a single HRM peak
| Number of replicates | Tm Mean ± SD (SE) | D’Agostino & Pearson omnibus normality test | Unpaired t-test with Welch’s correction | ||
|---|---|---|---|---|---|
| K2 | P-value | P-value | |||
| L. (L.) amazonensis | 16 | 83.91 ± 0.20 (0.05) | 0.1456 | 0.93 | < 0.001 |
| L. (L.) infantum | 114 | 84.44 ± 0.36 (0.03) | 1.28 | 0.53 | |
Abbreviations: T m high resolution melting temperature, SD standard deviation, SE standard error
