Table 3.

qPCR-ML and qPCR-ama results

Species	qPCR ML (Cq ± SD)	qPCR-ama (Cq ± SD)
	Undiluted	Undiluted	1:10	1:100	1:1,000	1:10,000
L. (L.) infantum MHOM/TN/80/IPT1	17.26 ± 0.19	negative	negative	negative	nt	nt
L. (L.) infantum MHOM/FR/78/LEM75	15.30 ± 0.41	nt	33.80 ± 0.33	37.92 ± 2.23	negative	negative
L. (L.) infantum MHOM/ES/81/BCN1	15.80 ± 0.77	33.70 ± 0.33	38.01 ± 1.36	negative	negative	nt
L. (L.) infantum MHOM/DZ/82/LIPA59	16.43 ± 0.47	33.03 ± 0.61	nt	nt	negative	negative
L. (L.) infantum MHOM/IT/93/ISS822	14.73 ± 0.42	33.89 ± 0.66	nt	nt	negative	negative
L. (L.) infantum MHOM/IT/86/ISS218	15.42 ± 1.03	32.44 ± 1.18	nt	nt	negative	negative
L. (L.) infantum Clinical isolate V2921	17.09 ± 0.12	29.08 ± 0.13	nt	nt	negativea	negative
L. (L.) infantum Clinical isolate 31u	14.30 ± 0.27	28.73 ± 0.78	nt	nt	negativea	negative
L. (L.) infantum Clinical isolate 49u	15.71 ± 0.78	30.02 ± 0.01	nt	nt	negative	negative
L. (L.) infantum Clinical isolate 791	14.88 ± 0.51	27.76 ± 0.07	nt	nt	38.81 ± 0.93	negative
L. (L.) infantum Clinical isolate 10816	13.53 ± 0.46	26.53 ± 0.11	nt	nt	36.67 ± 2.08	negative
L. (L.) amazonensis MHOM/BR/00/LTB0016	23.79 ± 0.25	17.97 ± 0.40	21.08 ± 0.23	25.00 ± 0.66	27.97 ± 1.21	31.49 ± 0.88
L. (L.) amazonensis IFLA/BR/67/PH8	34.42 ± 0.02	15.32 ± 0.01	19.33 ± 0.32	nt	nt	nt
L. (L.) amazonensis Clinical isolate	31.66 ± 0.19	20.87 ± 0.24	24.12 ± 0.14	nt	nt	nt
L. (V.) panamensis Clinical isolate	22.96 ± 0.28	35.57 ± 0.56	nt	negative	nt	nt
L. (V.) guyanensis Clinical isolate	29.71 ± 0.32	33.30 ± 0.17	36.78 ± 0.46	negative	nt	nt
L. (V.) braziliensis Clinical isolate	28.15 ± 0.85	37.87 ± 0.86	negative	negative	nt	nt
L. (V.) braziliensis MHOM/BR/75/M2904	26.07 ± 1.80	38.19 ± 0.04	negative	negative	nt	nt

Abbreviation: nt not tested

^aOne replicate negative