Acute adult MS alters motivation. At the conclusion of cognitive testing, motivation was again tested with the PR task. After this testing, animals were given acute access to MS for 7 d, during which maintenance 5-CSRTT was performed, before motivation was again assessed with PR. Acute MS increased the motivation of HFD-fed offspring and offspring that had previous supplementation of methyl donors early in life. Motivation remained unchanged in control offspring. (n: SC = 14, HFD = 11, SC + MS = 13, HFD + MS = 16.) NS, not significant. #P < 0.05 compared with 0.