Figure 5.

HFD-fed offspring have increased expression of CCL2 in the PFC which is attenuated by MS. At the conclusion of operant testing, RNA gene expression was measured in the PFC. A, B) Expression of 2 chemokines, CXCL10 (A) and CCL2 (B), correlated with median stimulus duration on the 5-CSRTT titration schedule. C) HFD-fed offspring have increased CCL2 gene expression; MS attenuated this increase. (n: SC = 13, HFD = 11, SC + MS = 13, HFD + MS = 15 in (A); animals that did not reach the titration schedule were left out of correlation analysis (B, C): n: SC = 11, HFD = 10, SC + MS = 13, HFD + MS = 15.) *P < 0.05 SC vs. HFD; ^P < 0.05 HFD vs. HFD + MS.