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. 2017 Feb 21;31(6):2393–2404. doi: 10.1096/fj.201601198R

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Figure 5. — Murine corneas blotted before Ac₄GalNAz incubation do not incorporate GalNAz. WT mouse eyes were untreated (control; left) or blotted immediately after enucleation and before 24 h incubation in Ac₄GalNAz (preblotted; right), then labeled with DIBAC-biotin and streptavidin–Alexa Fluor 488. Maximum intensity projections (top) and orthogonal views of volumes (bottom) revealed greatly reduced GalNAz incorporation and expression in blotted eyes compared to controls. Images are representative of 4 eyes per condition.