Figure 7.

Corneas of IL-1R^−/− mice show reduced GalNAz labeling compared to WT and MyD88^−/− mice. A) WT mouse corneas were labeled with GalNAz as previously described (left), then analyzed using combination of fluorescence intensity, object shape, and segmentation with CellProfiler software to generate pattern of GalNAz-labeled cells (center) and distinguish those cells independently of cell-to-cell heterogeneity in fluorescence intensity (right). B) Eyes from MyD88^−/− mice (left) looked similar to WT, whereas IL-1R^−/− mouse corneas showed disrupted (patchy) mucin labeling (center). WT corneas blotted before Ac₄GalNAz exposure showed little or no labeling (right). Representative processed images with cell outlines are shown. In total, 8 WT, 6 MyD88^−/−, 7 IL-1R^−/−, and 4 blotted corneas were analyzed (Fig. 8).