Estradiol supplementation abrogated EPO effect in weight change in OVA mice. A) Cumulative changes in body weight of placebo (p) or estradiol (e) pellet-supplemented OVA mice treated with PBS (P) or EPO (E), respectively (n = 6 in each group). B–H) Summary of changes in placebo or estradiol pellet-supplemented OVA mice with PBS (open bar) or EPO (solid bar) treatment on HFD for cumulative change in body weight (B), cumulative change in fat mass (C), lean mass (D), average food intake (E), Hct (F), fasting serum glucose levels (G), and fasting serum insulin levels (H) (n = 6 in each group). I, J) EpoR expression in pgWAT and ingWAT isolated from (I) sham-surgery mice (solid bar) and OVA mice (open bar) fed on HFD and from (J) OVA mice supplemented with placebo pellets (OVA_p; open bar) or estradiol pellets (OVA_e; solid bar) (n = 4–6 in each group). *P < 0.05; **P < 0.01; #P < 0.001.