Figure 1.

Transgenic introduction of the D^b class I molecule confers resistance to chronic TMEV infection in FVB mice. FVB and transgenic FVB/D^b mice were intracranially infected with TMEV. At 4 mo post-TMEV infection, FVB/D^b mice, and not wild-type FVB mice, had cleared TMEV infection. RT-PCR analysis of virus load was determined via amplification of the VP2 gene. Wild-type FVB and FVB/D^b mice were infected with TMEV. Brain homogenate was collected for RT-PCR analysis. Viral VP2 gene and β-actin mRNA concentrations in each sample were calculated, and relative gene expression was assessed by using the C_t method. The bar graph represents means and sem of 8 replicates in FVB and FVB/D^b mice and 2 replicates in FVB uninfected controls. *P < 0.05, Student’s t test.