Figure 2.
SOB influences EAE susceptibility of 2× MOG35-55 + CFA–immunized B6/J mice. A, C) 2×- and 1×-immunized mice were stratified by month of birth (MOB). Numbers in parentheses indicate number of animals. Significance of observed differences in incidence of 2- (A) and 1×- (C) immunized mice as a function of MOB were determined by using Fisher’s exact test with January (A) and December to January (C) as reference variables, respectively. Animals were considered affected if clinical scores ≥ 1 were apparent for at least 2 consecutive days. B, D) Significance of observed differences in disease severity of 2- (B) and 1×- (D) immunized mice as a function of MOB were determined by 1-way ANOVA followed by Dunn’s multiple comparison test with January (B) and December to January (D) as reference variables, respectively. A significant effect of MOB was detected with 2×-immunized mice (F = 5.4; P < 0.0001) but not for 1×-immunized mice (F = 1.0; P = 0.4). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P < 0.0001.