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. 2017 Mar 9;31(6):2686–2695. doi: 10.1096/fj.201601348R

Figure 4.

Figure 4.

NAC decreases Gαq palmitoylation in HUVECs and promotes EC function in a PLC-β–dependent fashion. A) S-acylation pull down assay demonstrates that, relative to total protein levels, NAC can decrease Gαq palmitoylation in a dose-dependent fashion and does not affect Caveolin1 or Rac1 palmitoylation in HUVECs. B, C) Gαq palmitoylation is decreased with maximal effect at 2 and 4 h after treatment (n = 3). D) Click-chemistry assay also demonstrates a significant decrease in Gαq palmitoylation (n = 3). E) EC monolayer scratches have increased closure after treatment with NAC 3 mM. Cotreatment with PLC-β inhibitor U73122 1 and 7 μM inhibited EC monolayer scratch closure at 4 and 6 h after treatment (n = 6). F) EC proliferation was inhibited with NAC 3 mM and U73122 1 μM cotreatment (n = 8). *P < 0.05, **P < 0.01.