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. 2017 Mar 3;31(6):2520–2532. doi: 10.1096/fj.201601077R

Figure 7.

Figure 7.

Asbestos-induced lung cell apoptosis and AEC mtDNA damage are increased in Sirt3−/− mice. A) Lungs from WT and Sirt3−/− mice were harvested 3 wk after intratracheal instillation of TiO2 or crocidolite asbestos, as described in Fig. 6, and subjected to CC-3 or SFTPC IHC on serial sections. Shown is a representative panel. Red arrows indicate double-positive (CC-3 and SFTPC) cells. B) Semiquantitative analysis of 220 cells at the BAD junctions show a significant increase in CC-3 positive cells in WT mice that were treated with crocidolite, which is further amplified in Sirt3−/− mice. C) Primary AT2 cells obtained from WT and Sirt3−/− mice were exposed to asbestos (25 µg/cm2) or H2O2 (200 µM) for 24 h; n = 3. D) mtDNA damage was assessed in AT2 cells that were isolated from WT and Sirt3−/− mice 3 wk after intratracheal instillation with a single dose of TiO2 or crocidolite asbestos as described above; n = 3. E) Hypothetical model. N.S., not significant. *P < 0.05 vs. control or TiO2, +P < 0.05 vs. Sirt3+/+.