Figure 1.

Hepatic DsbA-L levels are negatively correlated with obesity. A, B) mRNA (A) or protein (B) levels of DsbA-L in liver of male C57BL/6 mice (n = 8 per group) fed normal chow diet (NC) or HFD for 8 wk were detected by real-time qPCR or Western blot analysis. Data are normalized to actin for loading control. C) DsbA-L protein levels in liver of 2 mo old male db/db mice and control littermates. All values represent means ± sem. *P < 0.05, **P < 0.01 by Student’s t test. D) Expression levels of DsbA-L in human liver were determined by Western blot analysis. Pearson correlation coefficient was applied to show correlation between DsbA-L protein level and body mass index in human subjects.