Figure 4.

Liver-specific knockout of DsbA-L exacerbates diet-induced hepatosteatosis. A) Representative images of hematoxylin and eosin staining (top) and Oil Red O staining (bottom) of liver tissues from normal chow and HFD-fed DsbA-L^LKO mice and control littermates. Scale bars, 50 μm. B, C) Absolute liver weight (B) and percentage to body weight (C) of HFD-fed DsbA-L^LKO mice and control littermates (n = 5 per group). D, E) Absolute (D) and relative (E) TG levels were measured and presented (n = 5 per group). F) Expression levels of genes involved in lipogenesis, fatty acid oxidation, lipid uptake, and very low-density lipoprotein secretion in liver of DsbA-L^LKO mice and their floxed control littermates were determined by real-time qPCR and normalized to actin (n = 4 per group). All values represent means ± sem. *P < 0.05, **P < 0.01, ***P < 0.001 (1-way ANOVA).