Table 2.

Precision accuracy and repeatability (%RSD) of this method

Analytes	Intra-day RSD%			Inter-day RSD%	Accuracy (n = 9)		Repeatability (n = 6)
	Day 1	Day 2	Day 3
	Pa	Pa	Pa	Pa	Mean recovery	RSD%	RSD%
UV detector
Loganic acid	1.13	2.09	2.36	1.58	102.6 %	1.93	1.79
Swertiamarin	1.53	1.72	1.97	1.46	101.8 %	2.62	1.88
Gentiopicroside	1.74	1.85	2.27	1.38	98.7 %	1.77	1.69
Isoorientin	1.22	2.21	2.33	2.25	102.2 %	2.56	1.71
Isovitexin	1.27	1.85	1.76	1.64	97.3 %	2.13	2.07
MRM
Loganic acid	1.26	1.58	1.71	1.57	99.3 %	1.99	1.86
Swertiamarin	1.45	1.31	1.67	1.78	100.9 %	1.86	1.22
Sweroside	1.48	1.37	1.94	0.76	101.4	1.78	1.85
Isoorientin	0.95	1.08	1.88	1.21	98.7 %	2.14	1.29
Isovitexin	1.66	1.33	1.92	1.59	98.9 %	1.98	2.24

P_a: peak areas