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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Cell Biochem. 2016 Jun 23;118(2):237–251. doi: 10.1002/jcb.25628

Fig. 1.

Fig. 1

NUMB6 overexpression induces changes in DB-7 cell morphology. (A) Schematic representation of NUMB isoforms: isoform 4 (NUMB4) and isoform 6 (NUMB6). NUMB4 and 6 differ in respect to deletion within the proline-rich region (PRR) in NUMB6. PTB is the phospho-tyrosine binding domain. PRR is proline-rich region. (B) DB-7 cell lines stably expressing NUMB4-GFP, NUMB6-GFP or empty vector with GFP. NUMB4 or NUMB6 proteins expression was examined by Western blot (upper panel) and immunofluorescence (lower panel) measuring GFP staining. (C) NUMB6 induced EMT-like elongation in DB-7 cells. The representative fluorescence images of control GFP, NUMB4-GFP and NUMB6-GFP DB-7 cells cultured in the normal cell culture condition (upper panel) and in collagen type 1 substratum (lower panel). NUMB6 caused the redistribution of filamentous actin (F-actin) in DB-7 cells, as determined by F-actin red staining with rhodamine-phalloidin. Nuclei were stained DAPI in blue. Bars=15 μm.