Fig. 8.

Schematic model of NUMB6-induced EMT in the regulation of cancer cell migration and invasion. The interaction of NUMB6 with Akt signaling pathway by unknown mechanism mediates induction of Slug transcription factor, ultimately leading to EMT. We found that EMT activation by NUMB6 overexpression triggered DB-7 cells migratory and invasive behavior. Because Akt pathway has been shown to affect cancer cell migration and invasion, we showed that chemical inhibition of the Akt phosphorylation abolished Slug expression in NUMB6 DB-7 cells, which in turn displayed reduced migration and invasion in vitro.