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. Author manuscript; available in PMC: 2018 May 16.
Published in final edited form as: Curr Protoc Microbiol. 2017 May 16;45:4C.2.1–4C.2.15. doi: 10.1002/cpmc.27

Figure 1. Mini-Tn7 insertions at B. thailandensis E264 attTn7 sites.

Figure 1

The two attTn7 sites are located 3′ to glmS-1 (Bth_I0288/Bth_RS13655) on chromosome I and 3′ to glmS-2 (Bth_II0348/BTH_RS01805) on chromosome II. Primers (red arrows) that anneal to the Tn7L region of mini-Tn7 and either Bth_I0286 (Bth_RS13660) or Bth_II0349 (Bth_RS01810) are used to confirm attTn7 insertion (for primer sequences, see Basic Protocol 2). In this example based on insertion from pUC18T-miniTn7T-Km (Choi et al., 2005), nptII (conferring kanamycin-resistance) carried on mini-Tn7 is flanked by flp recombinase target (FRT) sequences for marker excision, leaving the multiple cloning site (MCS) intact.