| water | 14.3 μl |
| 5× GoTaq® buffer | 5.0 μl |
| 10 mM dNTP mix | 1.0 μl |
| Forward primer, 10 μm | 1.0 μl |
| Reverse primer, 10 μm | 1.0 μl |
| DMSO | 2.5 μl |
| GoTaq® polymerase | 0.2 μl |
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