Figure 4. Alteration of ALDH2 activity and toxic aldehyde by Alda-1 pretreatment after intestinal I/R.

(A) Expression of ALDH2 in various tissues was measured by Western blotting. Representative blots against ALDH2 and β-actin are shown. ALDH2 activity was observed in (B) lung and (C) intestine at 2 h after intestinal I/R or sham operation. Aldehyde accumulation was evaluated by determining the MDA and 4-HNE levels in the lung (D and F) and intestine (E and G). Data were expressed as means±S.D.s (n=5–6/group) and compared using one-way ANOVA and LSD tests. ^#P<0.05 vs DMSO and *P<0.05 vs sham.