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. 2017 Apr 10;47(5):872–879. doi: 10.1002/eji.201646775

Figure 2.

Figure 2

Robust cross‐priming of cytotoxic T cells following Dectin‐1 activation in vivo depends on Card9. WT and Card9−/− mice were injected s.c. with OVA protein in combination with different adjuvants (Curdlan or CpG). (A) Frequency of H‐2Kb‐SIINFEKL Tetramer+ (OVA‐specific) cytotoxic T cells in the peripheral blood was determined by flow cytometry. An asterisk without brackets indicates comparison to the WT “No adj.” group. (B) In vivo cytotoxic activity was measured by target cell elimination of fluorescently labeled, SIINFEKL peptide‐pulsed syngenic splenocytes. The graph shows mean specific target cell lysis in the draining LN of individual mice. (A and B) Each circle represents a single replicate. Data are pooled from two independent experiments (A) or show representative data from one of two identical experiments (B). * p < 0.05, ** p < 0.01, *** p < 0.001; one‐way ANOVA with Bonferroni posttests.