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. 2017 May 17;37(3):BSR20170168. doi: 10.1042/BSR20170168

Figure 2. AKT, p473 AKT and p308 AKT expression in MCF-7, MCF-7TAM and MDA-MB-231 cells.

Figure 2

(A) Western blot for AKT: proteins were isolated after 24-h treatments at the following concentrations: EGCG 25 μg/mL, IIF 15 μM in MCF-7 cells or 30 μM in MCF-7TAM and MDA-MB-231 cells and combined treatments of EGCG + IIF at the aforementioned concentrations. AKT protein expression was expressed as percentage of treated over control samples (CTR). Each bar represents the mean (± S.D.) of three independent experiments. n.s. not significant. *P<0.05; **P<0.01. (B,C) Western blot for p473AKT and p308 AKT: proteins were isolated after 24-h treatments at the following concentrations: EGCG 25 μg/mL, IIF μM in MCF-7 cells or 30 μM in MCF-7TAM and MDA-MB-231 cells and combined treatments EGCG + IIF at the aforementioned concentrations. p473AKT and p308AKT protein expression were expressed as percentage of treated over control samples (CTR). Each bar represents the mean (± S.D.) of three independent experiments. n.s., not significant. *P<0.05 ; **P<0.01.