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. 2017 May 17;37(3):BSR20170168. doi: 10.1042/BSR20170168

Figure 6. Zymography and scratch assay in MCF-7TAM cells.

Figure 6

(A) Zymography on MCF-7TAM cells: MMP-2 and MMP-9 activity was measured after 24-h treatments at the following concentrations: EGCG 25 μg/mL, IIF 15 μM in MCF-7 cells or 30 μM in MCF-7TAM and MDA-MB-231 cells and combined treatments of EGCG + IIF at the aforementioned concentrations. The gelatin zymogram showed MMP-2 (62 kDa) and MMP-9 (83 kDa) in serum-free conditioned media. CTR, control samples. (B) In vitro scratch assay on MCF-7TAM cells treated with EGCG 25 μg/mL + IIF 30 μM for 24 h and evaluated after 24, 48 and 72 h. Data are expressed as the number of cells per field. CTR, control samples. Each bar represents the mean (± S.D.) of three independent experiments. n.s., not significant. *P<0.05; **P<0.01. Original magnification ×20.