. 2017 Mar 14;31(3):954–959. doi: 10.1111/jvim.14683

Table 3.

Agreement among the transtracheal wash (TTW) and each of nasal swab (NS), guarded nasopharyngeal swab (NPS) or bronchoalveolar lavage (BAL) for identification of respiratory pathogens in 100 preweaned Holstein calves with respiratory disease

Pathogen	^aMethod	^bNo. Calves with Each Combination of Results				Percent Positive Agreement	Kappa (95% CI)	^c P
Pathogen	^aMethod	+/+	+/−	−/+	−/−	Percent Positive Agreement	Kappa (95% CI)	^c P
Mannheimia haemolytica	NS	16	0	4	80	88.9 (78.1, 99.7)	0.86 (0.74, 0.99)	.125
	NPS	15	1	2	82	90.9 (80.7, 100)	0.89 (0.77, 1.00)	1.00
	BAL	15	1	2	82	90.9 (80.7, 100)	0.89 (0.77, 1.00)	1.00
Pasteurella multocida	NS	57	2	3	38	95.8 (92.1, 99.5)	0.90 (0.81, 0.99)	1.00
	NPS	58	1	3	38	96.7 (93.4, 99.9)	0.92 (0.84, 1.00)	.625
	BAL	57	2	3	38	95.8 (92.1, 99.5)	0.90 (0.81, 0.99)	1.00
Mycoplasma spp.	NS	81	3	5	0	95.3 (92.0, 98.6)	−0.04 (−0.10, 0.01)	.727
	NPS	78	8	5	0	92.3 (88.1, 96.5)	−0.07 (−0.14, −0.01)	.581
	BAL	81	1	5	0	96.4 (93.6, 99.3)	−0.02 (−0.06, 0.02)	.219
Mycoplasma bovis	NS	41	7	1	39	91.1 (85.0, 97.3)	0.82 (0.70, 0.94)	.070
	NPS	41	4	3	35	92.1 (86.3, 97.9)	0.83 (0.71, 0.95)	1.00
	BAL	45	1	2	40	96.8 (93.1, 100)	0.93 (0.86, 1.00)	1.00
Bovine respiratory syncytial virus	NS	7	7	2	78	60.9 (37.3, 84.4)	0.56 (0.30, 0.81)	.180
	NPS	11	4	2	76	78.6 (61.8, 95.3)	0.75 (0.56, 0.94)	.688
	BAL	13	0	2	78	92.9 (83.0, 100)	0.92 (0.80, 1.00)	.500
Bovine coronavirus	NS	6	0	7	76	63.2 (37.8, 88.5)	0.59 (0.33, 0.86)	.016
	NPS	6	0	9	70	57.1 (31.9, 82.4)	0.52 (0.27, 0.78)	.004
	BAL	6	0	3	77	80.0 (57.8, 100)	0.78 (0.55, 1.00)	.250

NS, nasal swab; NPS, nasopharyngeal swab; BAL, bronchoalveolar lavage.

Dichotomous testing results for paired samples collected by TTW/and the comparison method, respectively. The total number of calves differs across pathogens due to culture contamination or indeterminate PCR results for at least sample of each pair compared.

P‐value for McNemar's exact test.