Table 6. The enzyme stability under different conditions of the pH as well as the used metal ions at 45 °C .
Run | Temperature (°C) | pH Ca2+ (mM) | Mn2+ (mM) | Activity (U.mL-1) | t1/2 (min) | |
1 2 3 4 5 6 7 |
45 45 45 45 45 45 45 |
8.0 8.0 10.0 8.0 8.0 6.0 8.0 |
10 10 10 10 0 10 20 |
5 0 5 15 5 5 5 |
93.1±0.8 79.3±2.0 58.5±0.9 77.7±1.2 69.2±2.1 45.8±2.2 68.1±1.4 |
267.2±18.4 251.2±24.2 180.6±26.7 256.6±11.3 205.7±18.4 147.8±19.7 256.2±10.6 |