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| Primers | Forward(5´-3´) | Reverse(5´-3´) | Tm | Product size |
|
Core Core-E1-E2 |
CCGGGATCCATGGGCACAAATCCTAAACCTCAAAG (BamHI) CCGGGATCCATGGGCACAAATCCTAAACCTCAAAG (BamHI) |
TGGAGCTCCTTAAGAGCAGAGACCGGAACGGTG(SacI, AflII) ATAAGCTTTGCTTCGGCCTGGCCCAACAAG(Hind III) |
55ºC 58ºC |
573bp 2238bp |
