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. 2016 Jun;14(2):13–18. doi: 10.15171/ijb.1266

Table 2. The effect of the different nitrogen sources on the C. indologenes MUT.2 growth and its extracellular polymeric substance (EPS) production during cultivation in shake-flasks for 96 hours at 30ºC.The highest cell mass, EPS and other related factors were observed when glutamic acid was used as nitrogen source .

Nitrogen sources Dry cell weight (g.L-1) EPS (g.L-1) EPS/CDW Final pH
Control*
Glycine
Glutamic acid
Aspartic acid
Proline
Ammonium
sulphate
Sodium nitrate
Meat extract
Yeast extract 		0.95
1.53
1.96
1.34
1.21
1.44

1.48
1.70
1.81 		1.21
4.95
8.87
3.51
3.92
5.32

4.35
6.78
7.17 		1.27
3.23
4.52
2.61
3.23
3.69

2.93
3.98
3.96 		6.07
6.51
6.61
6.38
5.96
5.81

5.49
6.32
6.41

*Control culture without any nitrogen source