Table 1. CCD of the experiments for DEE optimization utilizing DX-7 software .
Run | The number of freeze/thawing cycles | Eptifibatide (μmol) | DSPC (mM) | Cholesterol (mM) | Dipalmitoyl-GRGDSPA peptide (mM) | DEE (%) |
1 | 3 | 1.65 | 6.25 | 3.625 | 0.75 | 0.68 |
2 | 6 | 2.7 | 7 | 2.75 | 1.5 | 3.31 |
3 | 3 | 1.65 | 6.25 | 1.875 | 1.25 | 8.62 |
4 | 8 | 1.65 | 6.25 | 1.875 | 0.75 | 11.16 |
5 | 8 | 1.65 | 7.75 | 1.875 | 1.25 | 16.29 |
6 | 3 | 1.65 | 7.75 | 3.625 | 1.25 | 20.25 |
7 | 6 | 2.7 | 7 | 2.75 | 0.5 | 3.74 |
8 | 8 | 3.75 | 7.75 | 1.875 | 0.75 | 24.39 |
9 | 3 | 3.75 | 7.75 | 1.875 | 1.25 | 25.85 |
10 | 6 | 0.6 | 7 | 2.75 | 1 | 27.28 |
11 | 8 | 1.65 | 7.75 | 3.62 | 0.75 | 27.52 |
12 | 6 | 2.7 | 5.5 | 2.75 | 1 | 28.14 |
13 | 6 | 2.7 | 7 | 1 | 1 | 29.99 |
14 | 6 | 2.7 | 7 | 2.75 | 1 | 27.28 |
15 | 8 | 3.75 | 6.25 | 1.875 | 1.25 | 32.75 |
16 | 6 | 2.7 | 7 | 2.75 | 1 | 35.74 |
17 | 1 | 27 | 7 | 2.75 | 1 | 33.63 |
18 | 8 | 3.75 | 7.75 | 3.625 | 1.25 | 33.65 |
19 | 6 | 2.7 | 7 | 4.5 | 1 | 33.68 |
20 | 6 | 2.7 | 8.5 | 2.75 | 1 | 34.64 |
21 | 6 | 2.7 | 7 | 2.75 | 1 | 36.95 |
22 | 3 | 3.75 | 6.25 | 3.625 | 1.25 | 36.23 |
23 | 6 | 4.8 | 7 | 2.75 | 1 | 37.79 |
24 | 8 | 1.65 | 6.25 | 3.625 | 1.25 | 26.53 |
25 | 3 | 1.65 | 7.75 | 1.875 | 0.75 | 28.01 |
26 | 6 | 2.7 | 7 | 2.75 | 1 | 30.81 |
27 | 8 | 3.75 | 6.25 | 3.625 | 0.75 | 29.74 |
28 | 3 | 3.75 | 7.75 | 3.625 | 0.75 | 35.23 |
29 | 11 | 2.7 | 7 | 2.75 | 1 | 35.59 |
30 | 6 | 2.7 | 7 | 2.75 | 1 | 28.98 |
31 | 6 | 2.7 | 7 | 2.75 | 1 | 33.17 |
32 | 3 | 3.75 | 6.25 | 1.875 | 0.75 | 5.71 |