Fig. 7.

Different Cid paralogs evolve under different evolutionary pressures. (A) Summary of tests for positive selection performed on each Cid paralog. Tests that were statistically significant (P < 0.05) are indicated with an asterisk. For the McDonald–Kreitman (MK) test, Neutrality Index (N.I.) is also displayed. N.I. < 1 indicates an excess of nonsynonymous fixed differences between species and suggests positive selection. (B) A protein alignment of Cid5 from virilis group species. The variable, proline-rich region which was excluded from PAML tests for positive selection is highlighted in blue. (C) A schematic of a representative Cid protein, showing sites evolving under positive selection identified by Bayes Empirical Bayes analyses (posterior probability > 0.95).