Fig 4. Adenine nucleotide levels in the wild type in no P_i and in adk1Δ, aah1Δ and ado1Δ in high P_i.

(A) [ATP], [ADP], and [AMP] in wild type (WT) cells over time grown in no P_i medium. All the adenine nucleotide concentrations at each time point were normalized to those in 10 mM P_i. Note that the PHO pathway in no P_i is activated within 15 minutes. Adenine nucleotide levels at each time point were measured three times and the error bars in (A) are standard errors. (B) [ATP], [ADP], and [AMP] in WT, adk1Δ, aah1Δ, and ado1Δ in 10 mM P_i. Adenine nucleotide levels in the three mutants were measured two times and the error bars in (B) are standard errors.