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. 2017 May 17;12(5):e0176689. doi: 10.1371/journal.pone.0176689

Fig 3. Dro expression reduces intestinal stress response, immune and regeneration activity.

Fig 3

(A) Dro transcription in the gut of female TiGS2>Dro flies fed with 10 μg/ml RU at different ages. (B) Gut-specific expression of Dro reduces transcription of genes involved in stress response, immune and regeneration activity in the intestinal tract. Transcriptional analysis of different pathways in midguts of female TiGS2>Dro flies fed with 10 μg/ml RU at different ages. For details of the tested genes and pathways see main text. (A–B) Mean log10 of fold change is compared to controls of same age fed without RU (set to 0, not shown). n = number of replicates of five guts. X represents outliers (Grubbs test, see Materials and methods): –0.57 (pirk), –0.24 (Socs36E). Note that data in (A) and (B) originate from the same experiments. (C) Dro transcription in the gut reduces intestinal damage. Smurf analysis of female TiGS2>Dro flies fed ± 10 μg/ml RU at different ages. n = number of flies. Statistical tests: (A–B) one sample t-test, (C) Fisher´s exact test. Error bars represent the standard error of the mean. aos, argos; Dro, Drosocin; EGF, Epidermal growth factor signaling; Hsp70A, Heat-shock-protein-70A; IMD, Immune Deficiency pathway; Irc, Immune-regulated catalase; JAK-STAT, Janus kinase / Signal Transducer and Activator of Transcription pathway; JNK, c-Jun N-terminal kinase signaling; PGRP-LB, Peptidoglycan recognition protein LB; pirk, poor Imd response upon knock-in; puc, puckered; rho, rhomboid; ROS, Reactive oxygen species production; Socs36E, Suppressor of cytokine signaling at 36E; upd3, unpaired 3. Genotype was: w/y,w;UAS-Dro/+;TiGS2GeneSwitch-gal4/+ (TiGS2>Dro).