Figure 2.

Lipocalin 2 (LCN2) protein was markedly upregulated in the brain 24 h after intraventricular haemoglobin injection compared with saline controls. Immunohistochemistry staining demonstrated that LCN2 immunoreactivity was expressed in the periventricular area and cortex 24 h after intraventricular haemoglobin injection (A) compared with saline controls (B). Boxes show areas examined at higher magnification in the higher power micrographs on the right (scale bars=100 and 20 µm). LCN2 protein levels were upregulated in the periventricular area (C) and cortex (D) 24 h after intraventricular haemoglobin injection, as shown by western blot. The data points represent means±SD, n=3–5, ^**p<0.01 versus saline group.